Quantitative Investigation of bioappearance and metabolism of trigonelline and its bioactive roasting products in coffee brew
Organizational Data
- DRKS-ID:
- DRKS00004524
- Recruitment Status:
- Recruiting complete, study complete
- Date of registration in DRKS:
- 2013-03-12
- Last update in DRKS:
- 2022-03-24
- Registration type:
- Retrospective
Acronym/abbreviation of the study
No Entry
URL of the study
No Entry
Brief summary in lay language
In a human intervention study, roast coffee brew is administered to the participating healthy volunteers. The study aims at quantitative analysis of coffee derived compounds and selected metabolic parameters in the plasma, which are discussed to be involved in diabetes type 2 prevention, inflammation and activation of phase I/II enzyme systems. The control group consists of a similar healthy group of volunteers without intervention.
Brief summary in scientific language
Beside caffeine, the betaine trigonelline is the second most abundant alkaloid in raw coffee. This thermolabile compound is largely degraded during coffee roasting giving rise to a variety of pyridine-derivatives.1 One of these pyrolysis products is niacin, also known as vitamin B3. Since roast coffee contains a fairly high amount of niacin, consumption of the brew supplies a significant portion of the daily demand (~17 mg/d).2,3 A further quantitatively important roast product of trigonelline is the recently discovered N-methylpyridinium (NMP), which can reach concentrations of 20-40 mg/L and beyond in the roast coffee brew.4-6 In murine animal models, administration of NMP induced the activity of Phase I/II detoxification enzymes. NMP was subsequently suggested to excert “chemoprotective” properties in living systems.7 Recent research substantiated this hypothesis, as NMP was found to activate antioxidant-reponse-element (ARE-) dependent gene expression of several detoxification enzymes in human colon carcinoma cell line HT29.8 Trigonelline, the progenitor of NMP, is still highly abundant in roast coffee brew despite the heavy losses suffered during roasting. Based on its anti-diabetic properties in animal studies, trigonelline is discussed in the context of diabetes treatment in man.9-11 1 Viani, R.; Horman, I. (1974) Thermal Behaviour of Trigonelline J. Food Sci. 39, 1216-1217 2 Taguchi, H.; Sakaguchi, M.; Shimabayashi, Y. (1985) Trigonelline Content in Coffee Beans and the Thermal Conversion of Trigonelline into Nicotinic Acid during the Roasting of Coffee Beans. Agric. Biol. Chem. 49, 3467-3471 3 Experts Group on Vitamins and Minerals, Review of Niacin. London Food Standard Agency 2002; http://www.food.gov.uk/multimedia/pdfs/evm-01-11r.pdf 4 Stadler, R.H.; Varga, N.; Hau, J.; Vera, F.A.; Welti, D. (2002) Alkylpyridiniums. 1. Formation in model systems via thermal degradation of trigonelline. J. Agric. Food Chem. 50, 1192-1199 5 Stadler, R.H.; Varga, N.; Milo, C.; Schilter, B.; Vera, F.A.; Welti, D. (2002) Alkylpyridiniums. 2. Isolation and quantification in roasted and ground coffees. J. Agric. Food Chem. 50, 1200-1206; 6 Weiss, C.; Rubach, M.; Lang, R.; Seebach, E.; Blumberg, S.; Frank, O.; Hofmann, T.; Somoza, V. (2010) Measurement of the intracellular pH in human stomach cells: a novel approach to evaluate the gastric acid secretory potential of coffee beverages. J. Agric. Food Chem. 58, 1976-1985. 7 Somoza et al., 2003, J. Agric. Food Chem, 51, 6861-6869 8 Boettler, U.; Sommerfeld, K.; Volz, N.; Pahlke, G.; Teller, N.; Somoza, V.; Lang, R.; Hofmann, T.; Marko, D. (2011) Coffee constituents as modulators of Nrf2 nuclear translocation and ARE (EpRE)-dependent gene expression. J. Nutr. Biochem., 22, 426-440. 9 Yoshinari, O.; Sato, H.; Igarashi, K. (2009) Anti-diabetic effects of pumpkin and its compounds, trigonelline and nicotinic acid, on Goto-Kakizaki rats. Biosci. Biotechnol. Biochem. 73, 1033-1041. 10 Mishinksy, J.; Joseph, B.; Sulman, F.G. (1967) Hypoglycaemic effect of trigonelline. Lancet, 1311-1312. 11 Lang, R.; Yagar, E.F.; Eggers, R.; Hofmann, T. (2008) Quantitative Investigation of Trigonelline, Nicotinic Acid, and Nicotinamide in Foods, Urine, and Plasma by Means of LC-MS/MS and Stable Isotope Dilution Analysis. J. Agric. Food Chem. 56, 11114-11121
Health condition or problem studied
- Free text:
- healthy volunteers
- Healthy volunteers:
- Yes
Interventions, Observational Groups
- Arm 1:
- coffee-group ( once only 350ml coffee)
- Arm 2:
- control-group (once only 350ml water)
Endpoints
- Primary outcome:
- One of the aims is to collect quantitative plasma data on coffee constituents (in particular trigonelline and N-methylpyridinium) and metabolites of niacin by means of HPLC-MS/MS methods (stable isotope dilution assays), and analyse pharmacokinetic properties of the compounds. Measuring points are before and 15, 30, 45, 60, 120, 240, 360 und 480 min after coffee ingestion. First morning urine and urine is collected in a 2h time interval all day long.
- Secondary outcome:
- Further, quantitation of carnitine, saturated acylcarnitine (C12 up to C18), and unsaturated acylcarnitine (C18:1, C18:2, C18:3) (Measuring points are before and 15, 30, 45, 60, 120, 240, 360 und 480 min after ingestion of coffee or water) shall give a hint as to whether coffee ingestion affects lipid metabolism.
Study Design
- Purpose:
- Basic research/physiological study
- Allocation:
- Non-randomized controlled study
- Control:
-
- Other
- Phase:
- N/A
- Study type:
- Interventional
- Mechanism of allocation concealment:
- No Entry
- Blinding:
- No
- Assignment:
- Parallel
- Sequence generation:
- No Entry
- Who is blinded:
- No Entry
Recruitment
- Recruitment Status:
- Recruiting complete, study complete
- Reason if recruiting stopped or withdrawn:
- No Entry
Recruitment Locations
- Recruitment countries:
-
- Germany
- Number of study centers:
- Monocenter study
- Recruitment location(s):
-
- Other Freising
Recruitment period and number of participants
- Planned study start date:
- No Entry
- Actual study start date:
- 2009-09-15
- Planned study completion date:
- No Entry
- Actual Study Completion Date:
- 2012-12-14
- Target Sample Size:
- 28
- Final Sample Size:
- 27
Inclusion Criteria
- Sex:
- All
- Minimum Age:
- 20 Years
- Maximum Age:
- 40 Years
- Additional Inclusion Criteria:
- metabolically healthy volunteers
Exclusion Criteria
no consuming diseases
Addresses
Primary Sponsor
- Address:
- Else Kröner-Fresenius-Zentrum für Ernährungsmedizin der TU München Lehrstuhl für ErnährungsmedizinProf. Dr. med. Hans HaunerGregor-Mendel-Str. 285350 Freising-WeihenstephanGermany
- Telephone:
- 08161-712000
- Fax:
- 08161-712097
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
- Investigator Sponsored/Initiated Trial (IST/IIT):
- Yes
Contact for Scientific Queries
- Address:
- Lehrstuhl für Lebensmittelchemie und Molekulare SensorikDr.rer.nat. Roman LangLise-Meitner-Straße 3485354 Freising-WeihenstephanGermany
- Telephone:
- 08161-712903
- Fax:
- No Entry
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
Contact for Public Queries
- Address:
- Else Kröner-Fresenius-Zentrum für Ernährungsmedizin der TU MünchenDipl oec troph Yu-Mi LeeGregor-Mendel-Straße 285350 Freising-WeihenstephanGermany
- Telephone:
- 08161-712007
- Fax:
- No Entry
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
Principal Investigator
- Address:
- Lehrstuhl für Lebensmittelchemie und Molekulare SensorikDr.rer.nat. Roman LangLise-Meitner-Straße 3485354 Freising-WeihenstephanGermany
- Telephone:
- 08161-712903
- Fax:
- No Entry
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
Sources of Monetary or Material Support
Institutional budget, no external funding (budget of sponsor/PI)
- Address:
- Lehrstuhl für Lebensmittelchemie und molekulare Sensorik TU MünchenLise-Meitner-Straße 3485354 Freising-WeihenstephanGermany
- Telephone:
- 08161-712901
- Fax:
- No Entry
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
Institutional budget, no external funding (budget of sponsor/PI)
- Address:
- Else Kröner-Fresenius-Zentrum für Ernährungsmedizin der TU München Lehrstuhl für ErnährungsmedizinGregor-Mendel-Str. 285350 Freising-WeihenstephanGermany
- Telephone:
- 08161-712000
- Fax:
- 08161-712097
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
Ethics Committee
Address Ethics Committee
- Address:
- Ethikkommission der Fakultät für Medizin der Technischen Universität MünchenIsmaninger Str. 2281675 MünchenGermany
- Telephone:
- +49-89-41404371
- Fax:
- +49-89-41404199
- Contact per E-Mail:
- Contact per E-Mail
- URL:
- No Entry
Vote of leading Ethics Committee
- Vote of leading Ethics Committee
- Date of ethics committee application:
- 2009-07-10
- Ethics committee number:
- 2496/09
- Vote of the Ethics Committee:
- Approved
- Date of the vote:
- 2009-07-29
Further identification numbers
- Other primary registry ID:
- No Entry
- EudraCT Number:
- No Entry
IPD - Individual Participant Data
- Do you plan to make participant-related data (IPD) available to other researchers in an anonymized form?:
- No
- IPD Sharing Plan:
- No Entry
Study protocol and other study documents
- Study protocols:
- No Entry
- Study abstract:
- No Entry
- Other study documents:
- No Entry
- Background literature:
- No Entry
- Related DRKS studies:
- No Entry
Publication of study results
- Planned publication:
- No Entry
- Publikationen/Studienergebnisse:
- Development of a hydrophilic liquid interaction chromatography-high-performance liquid chromatography-tandem mass spectrometry based stable isotope dilution analysis and pharmacokinetic studies on bioactive pyridines in human plasma and urine after coffee consumption. Lang R, Wahl A, Skurk T, Yagar EF, Schmiech L, Eggers R, Hauner H, Hofmann T.
- Date of first publication of study results:
- No Entry
- DRKS entry published for the first time with results:
- No Entry
Basic reporting
- Basic Reporting / Results tables:
- No Entry
- Brief summary of results:
- No Entry